验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Jurkat cells, THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy SLC11A1 Ready-To-Use IHC Kit. SLC11A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32308-1-AP targets NRAMP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 |
| 别名 | SLC11A1, NRAMP 1 |
| 计算分子量 | 432 aa, 48 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC071165 |
| 基因名称 | SLC11A1 |
| Gene ID (NCBI) | 6556 |
| RRID | AB_3742575 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P49279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC11A1 also known as NRAMP1, belongs to the SLC11 family of solute carriers, which mediate the transport of divalent metals. NRAMP1 is a divalent metal importer specifically expressed in the membranes of late endosomes/lysosomes of professional phagocytes. It plays a crucial role in the defense against intracellular pathogens by depleting essential metal ions from endocytosed microbes, thereby preventing their proliferation(PMID: 24832660, PMID: 39824808).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NRAMP1 antibody 32308-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
