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验证数据展示

  • WB analysis using 32308-1-AP

    WB analysis using 32308-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 32308-1-AP

    WB analysis using 32308-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NRAMP1 Polyclonal antibody

NRAMP1 Polyclonal Antibody for WB, ELISA
Cat No. 32308-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

SLC11A1, NRAMP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy SLC11A1 Ready-To-Use IHC Kit. SLC11A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32308-1-AP targets NRAMP1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
别名 SLC11A1, NRAMP 1
计算分子量 432 aa, 48 kDa
观测分子量 60 kDa
GenBank蛋白编号BC071165
基因名称 SLC11A1
Gene ID (NCBI) 6556
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP49279
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC11A1 also known as NRAMP1, belongs to the SLC11 family of solute carriers, which mediate the transport of divalent metals. NRAMP1 is a divalent metal importer specifically expressed in the membranes of late endosomes/lysosomes of professional phagocytes. It plays a crucial role in the defense against intracellular pathogens by depleting essential metal ions from endocytosed microbes, thereby preventing their proliferation(PMID: 24832660, PMID: 39824808).

实验方案

Product Specific Protocols
WB protocol for NRAMP1 antibody 32308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols