验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, mouse spleen tissue |
| Positive IF/ICC detected in | ROS1728 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
55405-1-AP targets NR4A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 4, group A, member 3 |
| 别名 | TEC, NOR1, MINOR, CSMF, CHN |
| 计算分子量 | 70 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | NM_006981 |
| 基因名称 | NR4A3 |
| Gene ID (NCBI) | 8013 |
| RRID | AB_2881322 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92570 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR4A3 antibody 55405-1-AP | Download protocol |
| IF protocol for NR4A3 antibody 55405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Oxidative stress-induced EGR1 upregulation promotes NR4A3-mediated nucleus pulposus cells apoptosis in intervertebral disc degeneration |
