NR1H3 Monoclonal antibody
NR1H3 Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig and More (1)
Applications
IF/ICC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4A4F7
验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, HepG2 cells, MCF-7 cells, COLO 320 cells |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NR1H3 Ready-To-Use IHC Kit. NR1H3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
60134-1-Ig targets NR1H3 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Applications | IF/ICC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | NR1H3 fusion protein Ag6184 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nuclear receptor subfamily 1, group H, member 3 |
| Synonyms | Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC041172 |
| Gene Symbol | NR1H3 |
| Gene ID (NCBI) | 10062 |
| RRID | AB_2154887 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1H3 antibody 60134-1-Ig | Download protocol |
| IF protocol for NR1H3 antibody 60134-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Funct CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling. | ||
J Transl Med Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer |
