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验证数据展示

  • WB analysis using 15767-1-AP (same clone as 15767-1-PBS)

    WB analysis using 15767-1-AP (same clone as 15767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

    IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NQO2 antibody included
  • IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

    IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NQO2 antibody included
  • IP experiment of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

    IP experiment of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

    IP result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

    IF Staining of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NQO2 Polyclonal antibody, PBS Only

NQO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15767-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NQO2 Ready-To-Use IHC Kit. NQO2 primary antibody included.

产品信息

15767-1-PBS targets NQO2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8403

Product name: Recombinant human NQO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-231 aa of BC006096

Sequence: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NAD(P)H dehydrogenase, quinone 2
别名
计算分子量 231 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC006096
基因名称 NQO2
Gene ID (NCBI) 4835
RRIDAB_2236213
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16083
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

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