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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

    WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

    FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

NQO1 Monoclonal antibody, PBS Only

NQO1 Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 67240-1-PBS

产品说明书

CloneNo. 1E5G7

宿主/亚型

Mouse / IgG2a

种属反应性

rat, human

应用

Indirect ELISA, IHC, WB

别名

DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67240-1-PBS targets NQO1 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 rat, human
免疫原 NQO1 fusion protein Ag28933 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NAD(P)H dehydrogenase, quinone 1
别名 DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase
计算分子量 274 aa, 31 kDa
观测分子量 29-31 kDa
GenBank蛋白编号BC007659
基因名称 NQO1
Gene ID (NCBI) 1728
RRIDAB_2882519
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15559
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )