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  • Featured Product
  • KD/KO Validated

NQO1 Polyclonal antibody

NQO1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11451-1-AP

Print datasheet

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1

验证数据展示

  • WB analysis using 11451-1-AP

    WB analysis using 11451-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11451-1-AP

    IF Staining of HepG2 using 11451-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NQO1 antibody (11451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 11451-1-AP

    FC experiment of MCF-7 using 11451-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human NQO1 (11451-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HepG2 using 11451-1-AP

    WB analysis of HepG2 using 11451-1-AP

    WB result of NQO1 antibody (11451-1-AP, 1:1000) with si-control and si-NQO1 transfected HepG2 cells.

  • IHC staining of human colon cancer using 11451-1-AP

    IHC staining of human colon cancer using 11451-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11451-1-AP (NQO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 11451-1-AP

    IP experiment of HepG2 using 11451-1-AP

    IP result of anti-NQO1 (IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug.

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11451-1-AP targets NQO1 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB,IHC,IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, goat, chicken, rabbit, pig, bovine
Immunogen NQO1 fusion protein Ag2009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NAD(P)H dehydrogenase, quinone 1
Synonyms Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1
Calculated Molecular Weight 274 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC007659
Gene Symbol NQO1
Gene ID (NCBI) 1728
RRIDAB_2298729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

实验方案

Product Specific Protocols
WB protocol for NQO1 antibody 11451-1-APDownload protocol
IHC protocol for NQO1 antibody 11451-1-APDownload protocol
IF protocol for NQO1 antibody 11451-1-APDownload protocol
IP protocol for NQO1 antibody 11451-1-APDownload protocol
FC protocol for NQO1 antibody 11451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Authors - Kirill Bersuker
  • KO Validated
View Article
mouseWB

Sci Transl Med

Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease.

Authors - Yi Lu
View Article
humanWB

Mol Cell

Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.

Authors - Alessandra Fiore
View Article
mouseWB

Nat Commun

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Authors - Insup Choi
View Article
humanWB

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
View Article
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
View Article