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验证数据展示

  • WB analysis using 30848-1-AP

    WB analysis using 30848-1-AP

    mouse spinal cord tissue was subjected to SDS PAGE followed by western blot with 30848-1-AP (NPY5R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30848-1-AP

    IHC staining of mouse brain using 30848-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30848-1-AP (NPY5R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30848-1-AP

    IHC staining of mouse brain using 30848-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30848-1-AP (NPY5R antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of BxPC-3 using 30848-1-AP

    IF Staining of BxPC-3 using 30848-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using NPY5R antibody (30848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NPY5R Polyclonal antibody

NPY5R Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30848-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

NPY Y5 receptor, NPY5 R

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spinal cord tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30848-1-AP targets NPY5R in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5428

Product name: Recombinant human NPY5R protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 240-382 aa of BC042416

Sequence: CGLSNKENRLEENEMINLTLHPSKKSGPQVKLSGSHKWSYSFIKKHRRRYSKKTACVLPAPERPSQENHSRILPENFGSVRSQLSSSSKFIPGVPTCFEIKPEENSDVHELRVKRSVTRIKKRSRSVFYRLTILILVFAVSWM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuropeptide Y receptor Y5
别名 NPY Y5 receptor, NPY5 R
计算分子量 51 kDa
观测分子量50 kDa
GenBank蛋白编号BC042416
基因名称 NPY5R
Gene ID (NCBI) 4889
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ15761
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide Y receptor Y5 (NPY5R) is a G protein-coupled receptor (GPCR) that belongs to the neuropeptide Y receptor family and is primarily involved in regulating food intake, energy metabolism, and various physiological processes.

实验方案

Product Specific Protocols
IF protocol for NPY5R antibody 30848-1-APDownload protocol
WB protocol for NPY5R antibody 30848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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