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验证数据展示

  • IHC staining of human colon cancer using 25085-1-AP

    IHC staining of human colon cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 25085-1-AP

    IHC staining of human cervical cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 25085-1-AP

    IHC staining of human endometrial cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NPSR1 Polyclonal antibody

NPSR1 Polyclonal Antibody for IHC, ELISA
Cat No. 25085-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

neuropeptide S receptor 1, Neuropeptide S receptor, GPRA, GPR154, G protein coupled receptor 154

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25085-1-AP targets NPSR1 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18785

Product name: Recombinant human NPSR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-52 aa of BC132693

Sequence: MPANFTEGSFDSSGTGQTLDSSPVACTETVTFTEVVEGKEWGSFYYSFKTEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuropeptide S receptor 1
别名 neuropeptide S receptor 1, Neuropeptide S receptor, GPRA, GPR154, G protein coupled receptor 154
计算分子量 371 aa, 43 kDa
GenBank蛋白编号BC132693
基因名称 NPSR1
Gene ID (NCBI) 387129
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6W5P4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide S receptor 1 (NPSR1), previously known as GPRA and GPR154, is a G protein-coupled receptor (GPCR) that plays a significant role in various physiological processes. NPSR1 is primarily expressed in the bronchus, brain, and immune cells. It is also found in specific peripheral cell types such as monocytes/macrophages and neuroendocrine cells of the gut (PMID: 30711025).

实验方案

Product Specific Protocols
IHC protocol for NPSR1 antibody 25085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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