验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue, human hypothalamus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22311-1-AP targets NPVF in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuropeptide VF precursor |
| 别名 | C7orf9, FMRFamide related peptides, Neuropeptide RFRP 3, Neuropeptide VF, neuropeptide VF precursor, NPSF, NPVF, RFRP |
| 计算分子量 | 22 kDa |
| GenBank蛋白编号 | NM_022150 |
| 基因名称 | NPVF |
| Gene ID (NCBI) | 64111 |
| RRID | AB_3669395 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9HCQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human RFamide-related peptide gene, RFRP (also designated NPVF or C7orf9), is responsible for encoding three small neuropeptides designated NPSF, RFRP-1 RFRP-2 and RFRP-3 (NPVF). Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. This antibody can recognize neuropeptide VF precursor and neuropeptide NPSF.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPVF antibody 22311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |



