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验证数据展示

  • IHC staining of human kidney using 22311-1-AP

    IHC staining of human kidney using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 22311-1-AP

    IHC staining of human kidney using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 40x lens).

NPVF Polyclonal antibody

NPVF Polyclonal Antibody for IHC, ELISA
Cat No. 22311-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

C7orf9, FMRFamide related peptides, Neuropeptide RFRP 3, Neuropeptide VF, neuropeptide VF precursor, NPSF, NPVF, RFRP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22311-1-AP targets NPVF in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuropeptide VF precursor
别名 C7orf9, FMRFamide related peptides, Neuropeptide RFRP 3, Neuropeptide VF, neuropeptide VF precursor, NPSF, NPVF, RFRP
计算分子量 22 kDa
GenBank蛋白编号NM_022150
基因名称 NPVF
Gene ID (NCBI) 64111
RRIDAB_3669395
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9HCQ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human RFamide-related peptide gene, RFRP (also designated NPVF or C7orf9), is responsible for encoding three small neuropeptides designated NPSF, RFRP-1 RFRP-2 and RFRP-3 (NPVF). Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. This antibody can recognize neuropeptide VF precursor and neuropeptide NPSF.

实验方案

Product Specific Protocols
IHC protocol for NPVF antibody 22311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols