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验证数据展示

  • WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

    WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

    IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

    IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 27426-1-AP (same clone as 27426-1-PBS)

    IF Staining of C2C12 using 27426-1-AP (same clone as 27426-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27426-1-AP (same clone as 27426-1-PBS)

    IF Staining of HeLa using 27426-1-AP (same clone as 27426-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

NPPA Polyclonal antibody, PBS Only

NPPA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27426-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ANP, PND, ANF, ATFB6, Atrial natriuretic factor

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27426-1-PBS targets NPPA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26692

Product name: Recombinant human NPPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-153 aa of BC005893

Sequence: NPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRYRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 natriuretic peptide precursor A
别名 ANP, PND, ANF, ATFB6, Atrial natriuretic factor
计算分子量 153 aa, 17 kDa
观测分子量 19 kDa
GenBank蛋白编号BC005893
基因名称 NPPA
Gene ID (NCBI) 4878
RRIDAB_2880868
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01160
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.

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