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验证数据展示

  • IF Staining of HeLa using 85156-5-RR

    IF Staining of HeLa using 85156-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPM3 antibody (85156-5-RR, Clone: 242863G12 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85156-5-RR

    Affinity and Kinetic Characterization of 85156-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85156-5-RR against Human NPM3 were performed. The affinity constant is 1.98 nM.

NPM3 Recombinant antibody

NPM3 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 85156-5-RR

产品说明书

CloneNo. 242863G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

242863G12, nucleophosmin/nucleoplasmin, 3, Nucleoplasmin 3, Nucleoplasmin-3, PORMIN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85156-5-RR targets NPM3 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NPM3 fusion protein Ag2564 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleophosmin/nucleoplasmin, 3
别名 242863G12, nucleophosmin/nucleoplasmin, 3, Nucleoplasmin 3, Nucleoplasmin-3, PORMIN
计算分子量 178 aa, 19 kDa
GenBank蛋白编号BC054868
基因名称 NPM3
Gene ID (NCBI) 10360
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75607
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795).

实验方案

Product Specific Protocols
IF protocol for NPM3 antibody 85156-5-RRDownload protocol
Standard Protocols
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