验证数据展示
经过测试的应用
| Positive WB detected in | 5637 cells, Caco-2 cells, PANC-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87535-4-RR targets NPLOC4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2219 Product name: Recombinant human NPLOC4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 261-608 aa of BC025930 Sequence: DIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nuclear protein localization 4 homolog (S. cerevisiae) |
| 别名 | NPL4, Protein NPL4 |
| 计算分子量 | 608 aa, 68 kDa |
| 观测分子量 | 68-75 kDa |
| GenBank蛋白编号 | BC025930 |
| 基因名称 | NPLOC4 |
| Gene ID (NCBI) | 55666 |
| RRID | AB_3745606 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TAT6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NPLOC4 (NPL4 ubiquitin recognition factor) is an essential protein involved in numerous cellular processes, especially those related to the endoplasmic reticulum (ER) . It acts as a co-factor in the p97/VCP complex, which is vital for protein quality control and degradation via the ubiquitin-proteasome system. NPLOC4 plays a significant role in ER-associated degradation (ERAD), maintaining cellular homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPLOC4 antibody 87535-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
