验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse kidney tissue, Raji cells, rat brain tissue, rat kidney tissue |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16715-1-AP targets NPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | NPL fusion protein Ag10137 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) |
别名 | N-acetylneuraminate pyruvate-lyase, N-acetylneuraminate lyase, N acetylneuraminate lyase, EC:4.1.3.3, C1orf13 |
计算分子量 | 27 kDa, 35 kDa |
观测分子量 | 35 kDa |
GenBank蛋白编号 | BC058003 |
基因名称 | NPL |
Gene ID (NCBI) | 80896 |
RRID | AB_2154866 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BXD5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for NPL antibody 16715-1-AP | Download protocol |
IHC protocol for NPL antibody 16715-1-AP | Download protocol |
IF protocol for NPL antibody 16715-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |