验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66970-1-Ig targets Nephrin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19273 Product name: Recombinant human NPHS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 89-374 aa of BC156935 Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nephrosis 1, congenital, Finnish type (nephrin) |
| 别名 | NPHS1, 1D11H8, CNF, NPHN, NPHS 1 |
| 计算分子量 | 1241 aa, 135 kDa |
| GenBank蛋白编号 | BC156935 |
| 基因名称 | Nephrin |
| Gene ID (NCBI) | 4868 |
| RRID | AB_2882292 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O60500 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Nephrin antibody 66970-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro | ||
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
Genomics Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment | ||
Mol Cell Endocrinol Experimental study on renoprotective effect of intermedin on diabetic nephropathy. | ||
Exp Mol Pathol Urinary exosomes from patients with diabetic kidney disease induced podocyte apoptosis via microRNA-145-5p/Srgap2 and the RhoA/ROCK pathway | ||
Heliyon QiDiTangShen granules alleviates diabetic nephropathy podocyte injury: A network pharmacology study and experimental validation in vivo and vitro |