验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
Planning an IHC experiment? We recommend our IHCeasy NPHP3 Ready-To-Use IHC Kit. NPHP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22026-1-AP targets NPHP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16888 Product name: Recombinant human NPHP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC068082 Sequence: MGTASSLVSPAGGEVIEDTYGAGGGEACEIPVEVKPKARLLRNSFRRGAGAAAGAGPGSLPRGVGAGGLLGASFKSTGSSVPELEYAAAEYERLRKEYEIFRVSKNQELLSMGRREAKLDTENKRLRAELQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nephronophthisis 3 (adolescent) |
| 别名 | Nephrocystin-3, Nephrocystin 3, MKS7, KIAA2000 |
| 计算分子量 | 1330 aa, 151 kDa |
| 观测分子量 | 145-151 kDa |
| GenBank蛋白编号 | BC068082 |
| 基因名称 | NPHP3 |
| Gene ID (NCBI) | 27031 |
| RRID | AB_2878975 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z494 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| IHC protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| IP protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| WB protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. | ||
Am J Hum Genet ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition. | ||
Hum Mol Genet Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells. | ||
Int J Mol Sci Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. | ||
Mol Biol Cell Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia | ||
