NPHP3 Polyclonal antibody
NPHP3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | hTERT-RPE1 cells, MDCK cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 5 publications below |
产品信息
22026-1-AP targets NPHP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse |
| Immunogen | NPHP3 fusion protein Ag16888 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nephronophthisis 3 (adolescent) |
| Synonyms | KIAA2000, MKS7, Nephrocystin 3, NPHP3 |
| Calculated Molecular Weight | 1330 aa, 151 kDa |
| Observed Molecular Weight | 145-151 kDa |
| GenBank Accession Number | BC068082 |
| Gene Symbol | NPHP3 |
| Gene ID (NCBI) | 27031 |
| RRID | AB_2878975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z494 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| IHC protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| IF protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| IP protocol for NPHP3 antibody 22026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. | ||
Am J Hum Genet ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition. | ||
Hum Mol Genet Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells. | ||
Int J Mol Sci Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. | ||
Mol Biol Cell Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia | ||
