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NPEPL1 Polyclonal antibody

NPEPL1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17211-1-AP

Print datasheet

Synonyms

aminopeptidase like 1, KIAA1974, NPEPL1, Probable aminopeptidase NPEPL1

验证数据展示

  • WB analysis using 17211-1-AP

    WB analysis using 17211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17211-1-AP

    IHC staining of human kidney using 17211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 17211-1-AP

    IHC staining of human kidney using 17211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 17211-1-AP

    IF Staining of MCF-7 using 17211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NPEPL1 antibody (17211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17211-1-AP targets NPEPL1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen NPEPL1 fusion protein Ag10994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aminopeptidase-like 1
Synonyms aminopeptidase like 1, KIAA1974, NPEPL1, Probable aminopeptidase NPEPL1
Calculated Molecular Weight 523 aa, 56 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC020507
Gene Symbol NPEPL1
Gene ID (NCBI) 79716
RRIDAB_2267625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDH3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NPEPL1 antibody 17211-1-APDownload protocol
IHC protocol for NPEPL1 antibody 17211-1-APDownload protocol
IF protocol for NPEPL1 antibody 17211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols