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验证数据展示

  • IHC staining of human brain using 25517-1-AP

    IHC staining of human brain using 25517-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 25517-1-AP

    IHC staining of human brain using 25517-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 40x lens).

NPB Polyclonal antibody

NPB Polyclonal Antibody for IHC, ELISA
Cat No. 25517-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25517-1-AP targets NPB in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21877

Product name: Recombinant human NPB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 53-125 aa of BC126128

Sequence: ARRSQPYRGAEPPGGAGASPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuropeptide B
别名 hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7
计算分子量 125 aa, 13 kDa
观测分子量 ~10 kDa
GenBank蛋白编号BC126128
基因名称 NPB
Gene ID (NCBI) 256933
RRIDAB_2880114
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide B, also named as NPB, PPL7 and PPNBP, can be cleaved into two chains, Neuropeptide B-23 (NPB23, hL7) and Neuropeptide B-29 (NPB29, hL7C). It is involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway. It is widely expressed in central nervous system.

实验方案

Product Specific Protocols
IHC protocol for NPB antibody 25517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols