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验证数据展示

  • WB analysis of human brain using 13701-1-AP

    WB analysis of human brain using 13701-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13701-1-AP (NPAS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 13701-1-AP

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 13701-1-AP

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 40x lens).

NPAS1 Polyclonal antibody

NPAS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13701-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

bHLHe11, Member of PAS protein 5, MOP5, neuronal PAS domain protein 1, Neuronal PAS1, NPAS1, PASD5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13701-1-AP targets NPAS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4620

Product name: Recombinant human NPAS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 293-590 aa of BC039016

Sequence: APLAELPLHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLDKGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQLPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSGDEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTPGTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuronal PAS domain protein 1
别名 bHLHe11, Member of PAS protein 5, MOP5, neuronal PAS domain protein 1, Neuronal PAS1, NPAS1, PASD5
计算分子量 590 aa, 63 kDa
观测分子量 63-68 kDa
GenBank蛋白编号BC039016
基因名称 NPAS1
Gene ID (NCBI) 4861
RRIDAB_10644122
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NPAS1 antibody 13701-1-APDownload protocol
IHC protocol for NPAS1 antibody 13701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols