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验证数据展示

  • WB analysis of human brain using 13701-1-AP

    WB analysis of human brain using 13701-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13701-1-AP (NPAS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 13701-1-AP

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 13701-1-AP

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 40x lens).

NPAS1 Polyclonal antibody

NPAS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13701-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

bHLHe11, Member of PAS protein 5, MOP5, neuronal PAS domain protein 1, Neuronal PAS1, NPAS1, PASD5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13701-1-AP targets NPAS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NPAS1 fusion protein Ag4620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuronal PAS domain protein 1
别名 bHLHe11, Member of PAS protein 5, MOP5, neuronal PAS domain protein 1, Neuronal PAS1, NPAS1, PASD5
计算分子量 590 aa, 63 kDa
观测分子量 63-68 kDa
GenBank蛋白编号BC039016
基因名称 NPAS1
Gene ID (NCBI) 4861
RRIDAB_10644122
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NPAS1 antibody 13701-1-APDownload protocol
IHC protocol for NPAS1 antibody 13701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols