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验证数据展示

  • WB analysis using 66956-1-Ig

    WB analysis using 66956-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 66956-1-Ig

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens)..

  • IHC staining of human small intestine using 66956-1-Ig

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens)..

NOTUM Monoclonal antibody

NOTUM Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66956-1-Ig

产品说明书

CloneNo. 2F6D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

2F6D5, EC:3.1.1.98, hNOTUM, OK/SW-CL.30, Palmitoleoyl-protein carboxylesterase NOTUM

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, T-47D cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66956-1-Ig targets NOTUM in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6338

Product name: Recombinant human NOTUM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 76-430 aa of BC060882

Sequence: TMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCPTVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLGMLSNGS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 notum pectinacetylesterase homolog (Drosophila)
别名 2F6D5, EC:3.1.1.98, hNOTUM, OK/SW-CL.30, Palmitoleoyl-protein carboxylesterase NOTUM
计算分子量 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC060882
基因名称 NOTUM
Gene ID (NCBI) 147111
RRIDAB_2882279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6P988
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for NOTUM antibody 66956-1-IgDownload protocol
WB protocol for NOTUM antibody 66956-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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