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验证数据展示

  • WB analysis using 33280-1-AP

    WB analysis using 33280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33280-1-AP (NOTCH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 33280-1-AP

    IHC staining of mouse lung using 33280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 33280-1-AP

    IHC staining of mouse heart using 33280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOTCH4 Polyclonal antibody

NOTCH4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33280-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

hNotch4, INT3, Notch 4, Notch 4 extracellular truncation, Notch 4 intracellular domain

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse lung tissue, mouse heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33280-1-AP targets NOTCH4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36738

Product name: Recombinant human NOTCH4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 309-648 aa of BC140782

Sequence: DCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGTSCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLCQPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPCANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGQLCEVPLCAPNLCQPKQICKDQKDKANC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Notch homolog 4 (Drosophila)
别名 hNotch4, INT3, Notch 4, Notch 4 extracellular truncation, Notch 4 intracellular domain
计算分子量 2003 aa, 210 kDa
观测分子量210 kDa
GenBank蛋白编号BC140782
基因名称 NOTCH4
Gene ID (NCBI) 4855
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ99466
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurogenic locus notch homolog protein 4 (NOTCH4) is a receptor involved in the Notch signaling pathway, which is synthesized as a precursor that undergoes proteolytic cleavage to form an active receptor. NOTCH4 interacts with various ligands, leading to the release of the Notch intracellular domain (NICD), which translocates to the nucleus and regulates gene expression (PMID: 28794168; 19379690; 17148788). NOTCH4 is involved in various biological processes, including embryonic development, immune cell differentiation, and tumorigenesis (PMID: 24667410; 34925319).

实验方案

Product Specific Protocols
IHC protocol for NOTCH4 antibody 33280-1-APDownload protocol
WB protocol for NOTCH4 antibody 33280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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