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验证数据展示

  • WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

    WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

    WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

    IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

    IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 55114-1-AP (same clone as 55114-1-PBS)

    IF Staining of U2OS using 55114-1-AP (same clone as 55114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOTCH3 antibody (55114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 55114-1-AP (same clone as 55114-1-PBS)

    IP experiment of HeLa using 55114-1-AP (same clone as 55114-1-PBS)

    IP result of anti-NOTCH3 (IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NOTCH3 Polyclonal antibody, PBS Only

NOTCH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55114-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55114-1-PBS targets NOTCH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Notch homolog 3 (Drosophila)
别名 CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila)
计算分子量 244 kDa
观测分子量 250-260 kDa, 100 kDa
GenBank蛋白编号NM_000435
基因名称 NOTCH3
Gene ID (NCBI) 4854
RRIDAB_10858393
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM47
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

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