验证数据展示

WB analysis using 20116-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).
IP experiment of human placenta using 20116-1-AP
IP result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.
IF Staining of HepG2 using 20116-1-AP
Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse kidney using 20116-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 20116-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOSTRIN Polyclonal antibody

NOSTRIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 20116-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (13)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse heart tissue, human placenta tissue, mouse liver tissue, mouse lung tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human heart tissue, mouse testis tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	HepG2 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 6 publications below
IHC	See 4 publications below
IF	See 1 publications below

产品信息

20116-1-AP targets NOSTRIN in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag13989 Product name: Recombinant human NOSTRIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 122-428 aa of BC014189 Sequence: KWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEEGWWFGSLNGKKGHFPAAYVEELPSNAGNTATKA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	nitric oxide synthase trafficker
别名	eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog
计算分子量	506 aa, 58 kDa
观测分子量	64 kDa
GenBank蛋白编号	BC014189
基因名称	NOSTRIN
Gene ID (NCBI)	115677
RRID	AB_10666167
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q8IVI9
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.

实验方案

Product Specific Protocols
IF protocol for NOSTRIN antibody 20116-1-AP	Download protocol
IHC protocol for NOSTRIN antibody 20116-1-AP	Download protocol
IP protocol for NOSTRIN antibody 20116-1-AP	Download protocol
WB protocol for NOSTRIN antibody 20116-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
rat	WB	Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. Authors - Dejuan Li View Article
rat	WB	J Inflamm Res TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters Authors - Jufang Wang View Article
		Naunyn Schmiedebergs Arch Pharmacol Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway. Authors - Mohamed Abd-Elbaset View Article
rat	WB	Mol Med Rep Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Authors - Xiaoyan Hu View Article
rat	WB	Mol Med Rep UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats. Authors - Bingju Yan View Article
human	WB	Mol Med Rep Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. Authors - Bingju Yan View Article

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验证数据展示

WB analysis using 20116-1-AP

IHC staining of human heart using 20116-1-AP

IHC staining of human heart using 20116-1-AP

IP experiment of human placenta using 20116-1-AP

IF Staining of HepG2 using 20116-1-AP

IF Staining of mouse kidney using 20116-1-AP

IF Staining of mouse kidney using 20116-1-AP

NOSTRIN Polyclonal antibody

NOSTRIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway.

Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death.

UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.

Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.