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验证数据展示

  • WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

    WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

    WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • WB analysis using 85020-4-RR (same clone as 85020-4-PBS)

    WB analysis using 85020-4-RR (same clone as 85020-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85020-4-RR (eNOS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85020-4-RR

    Affinity and Kinetic Characterization of 85020-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85020-4-RR against Human NOS3 were performed. The affinity constant is 3.17 nM.

eNOS Recombinant monoclonal antibody, PBS Only

eNOS Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85020-4-PBS

产品说明书

CloneNo. 242543E10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

NOS3, 242543E10, cNOS, EC:1.14.13.39, ECNOS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85020-4-PBS targets eNOS in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag25712

Product name: Recombinant human NOS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of

Sequence: MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nitric oxide synthase 3 (endothelial cell)
别名 NOS3, 242543E10, cNOS, EC:1.14.13.39, ECNOS
观测分子量140 kDa
基因名称 eNOS
Gene ID (NCBI) 4846
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29474
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

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