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验证数据展示

  • WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

    WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

    WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85020-4-RR

    Affinity and Kinetic Characterization of 85020-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85020-4-RR against Human NOS3 were performed. The affinity constant is 3.17 nM.

NOS3 Recombinant antibody, PBS Only

NOS3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85020-4-PBS

产品说明书

CloneNo. 242543E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

eNOS, EC-NOS, ECNOS, EC:1.14.13.39, cNOS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85020-4-PBS targets NOS3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NOS3 fusion protein Ag25712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nitric oxide synthase 3 (endothelial cell)
别名 eNOS, EC-NOS, ECNOS, EC:1.14.13.39, cNOS
观测分子量140 kDa
基因名称 eNOS
Gene ID (NCBI) 4846
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29474
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.