验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, RAW 264.7 cells |
| Positive IHC detected in | mouse liver tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | LPS treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22226-1-AP targets iNOS in WB, IHC, IF, FC (Intra), CoIP, ELISA, Cell treatment, qPCR, Western Blot applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC (Intra), CoIP, Cell treatment, qPCR, Western Blot |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken |
| 免疫原 |
CatNo: Ag17567 Product name: Recombinant human NOS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC130283 Sequence: MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nitric oxide synthase 2, inducible |
| 别名 | NOS2, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase |
| 计算分子量 | 1153 aa, 131 kDa |
| 观测分子量 | 110-130 kDa, 65-70 kDa |
| GenBank蛋白编号 | BC130283 |
| 基因名称 | iNOS |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2879038 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35228 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for iNOS antibody 22226-1-AP | Download protocol |
| IHC protocol for iNOS antibody 22226-1-AP | Download protocol |
| WB protocol for iNOS antibody 22226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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