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NOP56 Polyclonal antibody

NOP56 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 18181-1-AP

Print datasheet

Synonyms

NOL5A, NOP56, Nucleolar protein 56, Nucleolar protein 5A

验证数据展示

  • WB analysis of NIH/3T3 using 18181-1-AP

    WB analysis of NIH/3T3 using 18181-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 18181-1-AP

    WB analysis using 18181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, A549 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18181-1-AP targets NOP56 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NOP56 fusion protein Ag12882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOP56 ribonucleoprotein homolog (yeast)
Synonyms NOL5A, NOP56, Nucleolar protein 56, Nucleolar protein 5A
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC104791
Gene Symbol NOP56
Gene ID (NCBI) 10528
RRIDAB_2152323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00567
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Box C/D RNA-protein complexes (RNPs) guide the 2'-O-methylation of nucleotides in eukaryotic ribosomal RNAs. The box C/D and C'/D' RNP subcomplexes are each assembled with three sRNP core proteins. The Nop56 core protein mediates crucial protein-protein interactions required for both sRNP assembly and the methyltransferase reaction by bridging the L7Ae and fibrillarin core proteins [PMID:22496443]. NOP56 is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2-prime-O-methylation of pre-ribosomal RNA (rRNA) during its maturation. It is predicted to function in an early to middle step in pre-rRNA processing [PMID:12777385].

实验方案

Product Specific Protocols
WB protocol for NOP56 antibody 18181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.

Authors - Miao Bai
View Article
mouseWB

iScience

BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.

Authors - Marlene Cervantes
View Article