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验证数据展示

  • WB analysis using 31969-1-AP

    WB analysis using 31969-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31969-1-AP (NOP16 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31969-1-AP

    IF Staining of A431 using 31969-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of MCF-7 using 31969-1-AP

    IF Staining of MCF-7 using 31969-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NOP16 Polyclonal antibody

NOP16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31969-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NOP16 Nucleolar Protein, Hypothetical Protein HSPC111, HSPC185, HSPC111, HBV pre-S2 trans-regulated protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, Hela cells, K-562 cells, MCF-7 cells, Raji cells
Positive IF/ICC detected inA431 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31969-1-AP targets NOP16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NOP16 fusion protein Ag36714 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOP16 nucleolar protein homolog (yeast)
别名 NOP16 Nucleolar Protein, Hypothetical Protein HSPC111, HSPC185, HSPC111, HBV pre-S2 trans-regulated protein 3
观测分子量23 kDa
GenBank蛋白编号BC040106
基因名称 NOP16
Gene ID (NCBI) 51491
RRIDAB_3670160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y3C1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOP16, also known as HSPC111, is found in the intracellular protein complex. It is a ribosomal protein that is transcriptionally regulated by the oncogene c-Myc. NOP16 may be involved in the completion of ribosome synthesis, therefore, reduced NOP16 expression in cancer cells may block many biological processes. Studies indicate that NOP16 is highly expressed in more than 20 types of cancers, including colorectal, breast, and hepatocellular carcinoma, and is an important target for clinical treatment of related diseases.

实验方案

Product Specific Protocols
WB protocol for NOP16 antibody 31969-1-APDownload protocol
IF protocol for NOP16 antibody 31969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols