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验证数据展示

  • WB analysis using 87529-1-RR (same clone as 87529-1-PBS)

    WB analysis using 87529-1-RR (same clone as 87529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87529-1-RR (NOP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87529-1-PBS in a different storage buffer formulation.

  • IHC staining of Human brain cancer（Astrocitoma） using 87529-1-RR (same clone as 87529-1-PBS)

    IHC staining of Human brain cancer（Astrocitoma） using 87529-1-RR (same clone as 87529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 87529-1-RR (NOP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87529-1-PBS in a different storage buffer formulation.

  • IHC staining of Human brain cancer（Astrocitoma） using 87529-1-RR (same clone as 87529-1-PBS)

    IHC staining of Human brain cancer（Astrocitoma） using 87529-1-RR (same clone as 87529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 87529-1-RR (NOP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87529-1-PBS in a different storage buffer formulation.

NOP10 Recombinant monoclonal antibody, PBS Only

NOP10 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87529-1-PBS

产品说明书

CloneNo. 252910G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

NOLA3, NOP 10, NOP10P

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87529-1-PBS targets NOP10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18718

Product name: Recombinant human NOP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-64 aa of BC063023

Sequence: MFLQYYLNEQGDRVYTLKKFDPMGQQTCSAHPARFSPDDKYSRHRITIKKRFKVLMTQQPRPVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOP10 ribonucleoprotein homolog (yeast)
别名 NOLA3, NOP 10, NOP10P
计算分子量 64 aa, 77 kDa
观测分子量10-12 kDa
GenBank蛋白编号BC063023
基因名称 NOP10
Gene ID (NCBI) 55505
RRIDAB_3745601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPE3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleolar protein 10 (NOP10) is a component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex and is essential for maintaining telomerase activity and RNA pseudouridylation. NOP10 is abnormally expressed in a variety of cancers such as breast cancer, pancreatic cancer, lung cancer, etc., and high expression is usually associated with poor prognosis(PMID: 33161513; 39538226).

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