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验证数据展示

  • WB analysis using 22769-1-AP

    WB analysis using 22769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22769-1-AP (NOP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 22769-1-AP

    IHC staining of human pancreas cancer using 22769-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22769-1-AP (NOP10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 22769-1-AP

    IHC staining of human pancreas cancer using 22769-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22769-1-AP (NOP10 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOP10 Polyclonal antibody

NOP10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22769-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NOP10P, NOP 10, NOLA3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22769-1-AP targets NOP10 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18718

Product name: Recombinant human NOP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-64 aa of BC063023

Sequence: MFLQYYLNEQGDRVYTLKKFDPMGQQTCSAHPARFSPDDKYSRHRITIKKRFKVLMTQQPRPVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOP10 ribonucleoprotein homolog (yeast)
别名 NOP10P, NOP 10, NOLA3
计算分子量 64 aa, 77 kDa
观测分子量10-12 kDa
GenBank蛋白编号BC063023
基因名称 NOP10
Gene ID (NCBI) 55505
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NPE3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleolar protein 10 (NOP10) is a component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex and is essential for maintaining telomerase activity and RNA pseudouridylation. NOP10 is abnormally expressed in a variety of cancers such as breast cancer, pancreatic cancer, lung cancer, etc., and high expression is usually associated with poor prognosis(PMID: 33161513; 39538226).

实验方案

Product Specific Protocols
IHC protocol for NOP10 antibody 22769-1-APDownload protocol
WB protocol for NOP10 antibody 22769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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