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NOMO1 Polyclonal antibody

NOMO1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17792-1-AP

Print datasheet

Synonyms

NODAL modulator 1, Nomo, NOMO1, PM5, pM5 protein

验证数据展示

  • WB analysis of mouse colon using 17792-1-AP

    WB analysis of mouse colon using 17792-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17792-1-AP

    IHC staining of human colon using 17792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

  • IHC staining of human colon using 17792-1-AP

    IHC staining of human colon using 17792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 17792-1-AP

    IF Staining of MCF-7 using 17792-1-AP

    Immunofluorescent analysis of MCF-7 cells, using NOMO1 antibody 17792-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A431 using 17792-1-AP

    IP experiment of A431 using 17792-1-AP

    IP result of anti-NOMO1 (IP:17792-1-AP, 4ug; Detection:17792-1-AP 1:1000) with A431 cells lysate 1520ug.

经过测试的应用

Positive WB detected inmouse colon tissue, A431 cells, COLO 320 cells, human brain tissue, mouse brain tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17792-1-AP targets NOMO1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NOMO1 fusion protein Ag11895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NODAL modulator 1
Synonyms NODAL modulator 1, Nomo, NOMO1, PM5, pM5 protein
Calculated Molecular Weight 1222 aa, 134 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC065535
Gene Symbol NOMO1
Gene ID (NCBI) 23420
RRIDAB_2152031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOMO1 antibody 17792-1-APDownload protocol
IHC protocol for NOMO1 antibody 17792-1-APDownload protocol
IF protocol for NOMO1 antibody 17792-1-APDownload protocol
IP protocol for NOMO1 antibody 17792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Clin Sci (Lond)

DLX3 promotes bone marrow mesenchymal stem cell proliferation through H19/miR-675 axis.

Authors - Na Zhao
View Article