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验证数据展示

  • WB analysis using 12043-1-AP

    WB analysis using 12043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12043-1-AP (NOL8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 12043-1-AP

    IP experiment of COLO 320 using 12043-1-AP

    IP result of anti-NOL8 (IP:12043-1-AP, 4ug; Detection:12043-1-AP 1:4000) with COLO 320 cells lysate 2560 ug.

NOL8 Polyclonal antibody

NOL8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12043-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

NOP132, NOL 8, C9orf34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells
Positive IP detected inCOLO 320 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12043-1-AP targets NOL8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2672

Product name: Recombinant human NOL8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 817-1147 aa of BC013788

Sequence: GEEWVKESMGKTSGKLFDSSDDDESDSEDDSNRFKIKPQFEGRAGQKLMDLQSHFGTDDRFRMDSRFLETDSEEEQEEVNEKKTAEEEELAEEKKKALNVVQSVLQINLSNSTNRGSVAAKKFKDIIHYDPTKQDHATYERKRDDKPKESKAKRKKKREEAEKLPEVSKEMYYNIAMDLKEIFQTTKYTSEKEEGTPWNEDCGKEKPEEIQDPAALTSDAEQPSGFTFSFFDSDTKDIKEETYRVETVKPGKIVWQEDPRLQDSSSEEEDVTEETDHRNSSPGEASLLEKETTRFFFFSKNDERLQGSDLFWRGVGSNMSRNSWEARTTNLRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar protein 8
别名 NOP132, NOL 8, C9orf34
计算分子量 132 kDa
观测分子量 150 kDa
GenBank蛋白编号BC013788
基因名称 NOL8
Gene ID (NCBI) 55035
RRIDAB_2152001
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ76FK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOL8 (Nucleolar Protein 8) plays an essential role in the survival of diffuse-type gastric cancer cells,and knocking down NOL8 promoted cell apoptosis. The structure analysis suggested that NOL8 contains a RNA binding motif, which was consistent with the study that NOL8 interacted with DEAD-box RNA helicase

实验方案

Product Specific Protocols
WB protocol for NOL8 antibody 12043-1-APDownload protocol
IP protocol for NOL8 antibody 12043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols