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验证数据展示

  • WB analysis of HEK-293 using 18424-1-AP

    WB analysis of HEK-293 using 18424-1-AP

    WB result of NOL7 antibody (18424-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOL7 transfected HEK-293 cells.

  • WB analysis using 18424-1-AP

    WB analysis using 18424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18424-1-AP (NOL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

NOL7 Polyclonal antibody

NOL7 Polyclonal Antibody for WB, ELISA
Cat No. 18424-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C6orf90, NOP27, Nucleolar protein 7, nucleolar protein 7, 27kDa, Nucleolar protein of 27 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18424-1-AP targets NOL7 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NOL7 fusion protein Ag13272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar protein 7, 27kDa
别名 C6orf90, NOP27, Nucleolar protein 7, nucleolar protein 7, 27kDa, Nucleolar protein of 27 kDa
计算分子量 257 aa, 29 kDa
观测分子量38 kDa
GenBank蛋白编号BC023517
基因名称 NOL7
Gene ID (NCBI) 51406
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOL7 (nucleolar protein 7) is localized in both the nucleolus and nucleoplasm, with its nucleolar localization being RNA-dependent. Its function in the nucleolus is likely related to maintaining nucleolar structure and cell growth, while its role in the nucleoplasm may be associated with anti-angiogenic effects. Additionally, NOL7 is involved in the accumulation of early pre-rRNA and the processing of pre-18S rRNA, which are crucial for maintaining nucleolar function and cell growth. The expression of NOL7 is regulated by various transcription factors, including c-myc and RXRα. In cervical cancer, NOL7 transcription is also positively regulated by the RB tumor suppressor gene. The NOL7 gene encodes three distinct splice variants, each with different intracellular localizations that may affect their functions.

实验方案

Product Specific Protocols
WB protocol for NOL7 antibody 18424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols