NOL11 Recombinant antibody
NOL11 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240371B5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HT-29 cells, SW480 cells, A431 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83391-6-RR targets NOL11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | NOL11 fusion protein Ag11783 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | nucleolar protein 11 |
Synonyms | Nucleolar protein 11, L14, 240371B5 |
Calculated Molecular Weight | 719 aa, 81 kDa |
Observed Molecular Weight | 81 kDa |
GenBank Accession Number | BC064404 |
Gene Symbol | NOL11 |
Gene ID (NCBI) | 25926 |
RRID | AB_3671045 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9H8H0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NOL11 antibody 83391-6-RR | Download protocol |
IF protocol for NOL11 antibody 83391-6-RR | Download protocol |
FC protocol for NOL11 antibody 83391-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |