Noggin Polyclonal antibody

Noggin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14772-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA

NOG, Noggin, SYM1, SYNS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14772-1-AP targets Noggin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse, hamster
免疫原 Noggin fusion protein Ag6586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 noggin
别名 NOG, Noggin, SYM1, SYNS1
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC034027
基因名称 Noggin
Gene ID (NCBI) 9241
RRIDAB_2151671
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14.

What is the molecular weight of Noggin? Is Noggin post-translationally modified?

The molecular weight of Noggin is 26 kDa. Noggin is secreted as a covalently linked glycosylated homodimer (PMID: 12478285).

What is the subcellular localization of Noggin?

Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with HSPG (PMID: 11706034).

What is the tissue expression pattern of Noggin?

In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles (PMID: 21256973).

What is the use of Noggin in cell culture?

Noggin can be used for maintaining the pluripotency of human embryonic stem cells (PMID: 19500111).


实验方案

Product Specific Protocols
WB protocol for Noggin antibody 14772-1-APDownload protocol
IHC protocol for Noggin antibody 14772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

PMID: 29415997

Authors - Shu-Fen Li
hamsterIHC

Birth Defects Res

Cigarette smoke induced neural tube defects by down-regulating noggin expression.

Authors - Chuanhui Yin
mouse

Microbiome

Desulfovibrio vulgaris interacts with novel gut epithelial immune receptor LRRC19 and exacerbates colitis

Authors - Runxiang Xie
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