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验证数据展示

  • IHC staining of mouse skin using 30628-1-AP

    IHC staining of mouse skin using 30628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30628-1-AP (NOD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 30628-1-AP

    IHC staining of mouse skin using 30628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30628-1-AP (NOD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30628-1-AP

    IF Staining of HeLa using 30628-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NOD2 antibody (30628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NOD2 Polyclonal antibody

NOD2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

CARD15, Caspase recruitment domain-containing protein 15, Nucleotide-binding oligomerization domain-containing protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30628-1-AP targets NOD2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NOD2 fusion protein Ag33771 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleotide-binding oligomerization domain containing 2
别名 CARD15, Caspase recruitment domain-containing protein 15, Nucleotide-binding oligomerization domain-containing protein 2
计算分子量 115 kDa
GenBank蛋白编号NM_001293557
基因名称 NOD2
Gene ID (NCBI) 64127
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).

实验方案

Product Specific Protocols
IHC protocol for NOD2 antibody 30628-1-APDownload protocol
IF protocol for NOD2 antibody 30628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols