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验证数据展示

  • WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 88267-1-RR (same clone as 88267-1-PBS)

    IHC staining of human placenta using 88267-1-RR (same clone as 88267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88267-1-RR (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NOC2L antibody included
  • IF Staining of U2OS using 88267-1-RR (same clone as 88267-1-PBS)

    IF Staining of U2OS using 88267-1-RR (same clone as 88267-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOC2L antibody (88267-1-RR, Clone: 260756G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03442-1

    Cytometric bead array sample test of MP03442-1

    The mean NOC2L concentration was determined to be 26.5 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load, 26.8 ng/mL in HT-1080 cell extract based on a 1.0 mg/mL extract load, 20.3 ng/mL in U2OS cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03442-1

    Cytometric bead array standard curve of MP03442-1

    Cytometric bead array standard curve of MP03442-1, NOC2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88267-1-PBS. Detection antibody: 88267-2-PBS. Standard: Ag26952. Range: 0.156-20 ng/mL.

  • Affinity of 88267-1-RR

    Affinity of 88267-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88267-1-RR against Human NOC2L were performed. The affinity constant is 0.413 nM.

NOC2L Recombinant monoclonal antibody, PBS Only (Capture)

NOC2L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 88267-1-PBS

产品说明书

CloneNo. 260756G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NOC2L Ready-To-Use IHC Kit. NOC2L primary antibody included.

产品信息

88267-1-PBS targets NOC2L as part of a matched antibody pair:

MP03442-1: 88267-1-PBS capture and 88267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleolar complex associated 2 homolog (S. cerevisiae)
别名 NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog
计算分子量 749 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC003555
基因名称 NOC2L
Gene ID (NCBI) 26155
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3T9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

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