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验证数据展示

  • Cytometric bead array sample test of MP51874-1

    Cytometric bead array sample test of MP51874-1

    Cytometric bead array sample test of MP51874-1, NOB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66048-1-PBS. Detection antibody: 66048-3-PBS.

  • WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51874-1

    Cytometric bead array standard curve of MP51874-1

    Cytometric bead array standard curve of MP51874-1, NOB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66048-1-PBS. Detection antibody: 66048-3-PBS. Standard:Ag17907. Range: 0.781-100 ng/mL.

NOB1 Monoclonal antibody, PBS Only (Capture)

NOB1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66048-1-PBS

产品说明书

CloneNo. 8C11A12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

ART 4, ART4, EC:3.1.-.-, MST158, MSTP158

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66048-1-PBS targets NOB1 as part of a matched antibody pair:

MP51874-1: 66048-1-PBS capture and 66048-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17907

Product name: Recombinant human NOB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 36-293 aa of BC000050

Sequence: SSFMFWRNPLPNIDHELQELLIDRGEDVPSEEEEEEENGFEDRKDDSDDDGGGWITPSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSRNPKVLNPRGLRYSLPTPKGGKYAINPHLTEDQRFPQLRLSQKARQKTNVFAPDYIAGVSPFVENDISSRSATLQVRDSTLGAGRRRLNPNASRKKFVKKR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
别名 ART 4, ART4, EC:3.1.-.-, MST158, MSTP158
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC000050
基因名称 NOB1
Gene ID (NCBI) 28987
RRIDAB_11042447
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULX3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

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