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验证数据展示

  • IHC staining of human colon cancer using 84876-9-RR (same clone as 84876-9-PBS)

    IHC staining of human colon cancer using 84876-9-RR (same clone as 84876-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84876-9-RR (NNMT antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84876-9-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84876-9-RR (same clone as 84876-9-PBS)

    IHC staining of human colon cancer using 84876-9-RR (same clone as 84876-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84876-9-RR (NNMT antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84876-9-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84876-9-RR (same clone as 84876-9-PBS)

    IF Staining of HepG2 using 84876-9-RR (same clone as 84876-9-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NNMT antibody (84876-9-RR, Clone: 242394A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84876-9-PBS in a different storage buffer formulation.

  • WB analysis using 84876-9-RR (same clone as 84876-9-PBS)

    WB analysis using 84876-9-RR (same clone as 84876-9-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84876-9-RR (NNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84876-9-PBS in a different storage buffer formulation.

  • Affinity of 84876-9-RR

    Affinity of 84876-9-RR

    Biolayer interferometry (BLI) kinetic assays of 84876-9-RR against Human NNMT were performed. The affinity constant is 10.3 nM.

NNMT Recombinant monoclonal antibody, PBS Only

NNMT Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84876-9-PBS

产品说明书

CloneNo. 242394A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.1.1.1, Nicotinamide N-methyltransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84876-9-PBS targets NNMT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7197

Product name: Recombinant human NNMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC000234

Sequence: MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nicotinamide N-methyltransferase
别名 EC:2.1.1.1, Nicotinamide N-methyltransferase
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000234
基因名称 NNMT
Gene ID (NCBI) 4837
RRIDAB_3743801
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP40261
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091). It plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PMID: 23455543; PMID: 26571212; PMID: 31043742).

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