验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, SH-SY5Y cells |
| Positive IHC detected in | human pituitary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26905-1-AP targets Neuronatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25408 Product name: Recombinant human NNAT protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 40-81 aa of BC001768 Sequence: NPPGTQPIARSEVFRYSLQKLAYTVSRTGRQVLGERRQRAPN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuronatin |
| 别名 | neuronatin, NNAT, Peg5 |
| 计算分子量 | 9 kDa |
| 观测分子量 | 9 kDa |
| GenBank蛋白编号 | BC001768 |
| 基因名称 | NNAT |
| Gene ID (NCBI) | 4826 |
| RRID | AB_2880678 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16517 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neuronatin antibody 26905-1-AP | Download protocol |
| IHC protocol for Neuronatin antibody 26905-1-AP | Download protocol |
| IF protocol for Neuronatin antibody 26905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Characterizing SERCA Function in Murine Skeletal Muscles after 35-37 Days of Spaceflight. | ||
Front Cardiovasc Med MiR-3064 in Epicardial Adipose-Derived Exosomes Targets Neuronatin to Regulate Adipogenic Differentiation of Epicardial Adipose Stem Cells. | ||
FEBS Lett Neuronatin promotes SERCA uncoupling and its expression is altered in skeletal muscles of high-fat diet-fed mice. | ||
J Toxicol Sci Lidocaine prevents breast cancer growth by targeting neuronatin to inhibit nerve fibers formation. | ||
J Biol Chem Low dose lithium supplementation promotes adipose tissue browning and sarco(endo)plasmic reticulum Ca2+ ATPase uncoupling in muscle |
