验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse skeletal muscle tissue |
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
13236-1-AP targets NMNAT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3965 Product name: Recombinant human NMNAT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC034374 Sequence: MYQVIQGIISPVNDTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHHHSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKDAHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEISATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGKTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nicotinamide nucleotide adenylyltransferase 3 |
| 别名 | EC:2.7.7.1, EC:2.7.7.18, FKSG76, NaMN adenylyltransferase 3, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 |
| 计算分子量 | 215 aa, 24 kDa |
| 观测分子量 | 24-28 kDa |
| GenBank蛋白编号 | BC034374 |
| 基因名称 | NMNAT3 |
| Gene ID (NCBI) | 349565 |
| RRID | AB_2877927 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96T66 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NMNAT3 antibody 13236-1-AP | Download protocol |
| WB protocol for NMNAT3 antibody 13236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice. | ||
Food Funct β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 | ||
Biosens Bioelectron Quantitative dynamics of intracellular NMN by genetically encoded biosensor | ||
Nat Cardiovasc Res Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm |
