验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28493-1-AP targets NMNAT1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag29584 Product name: Recombinant human NMNAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 52-152 aa of BC014943 Sequence: GDAYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQDSSQKKSLEPKTKAVPKVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nicotinamide nucleotide adenylyltransferase 1 |
| 别名 | NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC014943 |
| 基因名称 | NMNAT1 |
| Gene ID (NCBI) | 64802 |
| RRID | AB_3086056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NMNAT1 antibody 28493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |