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验证数据展示

  • WB analysis using 31096-1-AP

    WB analysis using 31096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31096-1-AP (NMI antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 31096-1-AP

    WB analysis of HeLa using 31096-1-AP

    WB result of NMI antibody (31096-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMI transfected HeLa cells.

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NMI Polyclonal antibody

NMI Polyclonal Antibody for WB, ELISA
Cat No. 31096-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor, N-myc-interactor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31096-1-AP targets NMI in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag34759

Product name: Recombinant human NMI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC001268

Sequence: MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-myc (and STAT) interactor
别名 N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor, N-myc-interactor
计算分子量 35 kDa
观测分子量37 kDa
GenBank蛋白编号BC001268
基因名称 NMI
Gene ID (NCBI) 9111
RRIDAB_3669853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13287
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.

实验方案

Product Specific Protocols
WB protocol for NMI antibody 31096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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