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验证数据展示

  • IHC staining of human brain using 26449-1-AP

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 26449-1-AP

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse embryo using 26449-1-AP

    WB analysis of mouse embryo using 26449-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 26449-1-AP (NME7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NME7 Polyclonal antibody

NME7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26449-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

NDK 7, NDP kinase 7, NME7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

26449-1-AP targets NME7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag24918

Product name: Recombinant human NME7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC006983

Sequence: MNHSERFVFIAEWYDPNASLLRRYELLFYPGDGSVEMHDVKNHRTFLKRTKYDNLHLEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)
别名 NDK 7, NDP kinase 7, NME7
计算分子量 376 aa, 42 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC006983
基因名称 NME7
Gene ID (NCBI) 29922
RRIDAB_2880517
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5B8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME7, also named as NDK7, nm23-H7, plays the major role in the synthesis of nucleoside triphosphates other than ATP. NME7 has two isoforms with MW 38-42 kDa.

实验方案

Product Specific Protocols
WB protocol for NME7 antibody 26449-1-APDownload protocol
IHC protocol for NME7 antibody 26449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Life Sci Alliance

NME7 maintains primary cilium assembly, ciliary microtubule stability, and Hedgehog signaling

Authors - Menghui Ji
View Article