NME5 Polyclonal antibody
NME5 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human kidney tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12923-1-AP targets NME5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | NME5 fusion protein Ag3610 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase) |
| Synonyms | IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog |
| Calculated Molecular Weight | 212 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC026182 |
| Gene Symbol | NME5 |
| Gene ID (NCBI) | 8382 |
| RRID | AB_2152973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NME5 antibody 12923-1-AP | Download protocol |
| IHC protocol for NME5 antibody 12923-1-AP | Download protocol |
| IP protocol for NME5 antibody 12923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Hazard Mater TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis. | ||
Int J Cancer Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma. | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice |
