验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | rat brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
28571-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag29423 Product name: Recombinant human NMDAR2A/GRIN2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1000-1127 aa of NM_000833 Sequence: VSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamate receptor, ionotropic, N-methyl D-aspartate 2A |
| 别名 | GRIN2A, NMDAR2A, glun2a, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate receptor ionotropic, NMDA 2A |
| 计算分子量 | 165 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | NM_000833 |
| 基因名称 | GRIN2A |
| Gene ID (NCBI) | 2903 |
| RRID | AB_2881171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12879 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| IHC protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| WB protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Behav Brain Res Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice | ||
Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 | ||
Neurochem Res Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression | ||
J Ethnopharmacol Anticonvulsant effect of Stachydrine on pentylenetetrazole-induced kindling seizure mouse model via Notch and NMDAR signaling pathways | ||
Neuron The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways |
