验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16060-1-AP targets NMD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8894 Product name: Recombinant human NMD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 154-503 aa of BC013317 Sequence: KAVIQVRQKTLHKKTFYYLEQLILKYGMHQNTLRIKEIHDGLDFYYSSKQHAQKMVEFLQCTVPCRYKASQRLISQDIHSNTYNYKSTFSVEIVPICKDNVVCLSPKLAQSLGNMNQICVCIRVTSAIHLIDPNTLQVADIDGSTFWSHPFNSLCHPKQLEEFIVMECSIVQDIKRAAGAGMISKKHTLGEVWVQKTSEMNTDKQYFCRTHLGHLLNPGDLVLGFDLANCNLNDEHVNKMNSDRVPDVVLIKKSYDRTKRQRRRNWKLKELARERENMDTDDERQYQDFLEDLEEDEAIRKNVNIYRDSAIPVESDTDDEGAPRISLAEMLEDLHISQDATGEEGASMLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NMD3 homolog (S. cerevisiae) |
| 别名 | NMD 3, hNMD3, CGI-07, CGI07, CGI 07 |
| 计算分子量 | 503 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC013317 |
| 基因名称 | NMD3 |
| Gene ID (NCBI) | 51068 |
| RRID | AB_2282830 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96D46 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NMD3 antibody 16060-1-AP | Download protocol |
| IHC protocol for NMD3 antibody 16060-1-AP | Download protocol |
| IP protocol for NMD3 antibody 16060-1-AP | Download protocol |
| WB protocol for NMD3 antibody 16060-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Synthesis of portimines reveals the basis of their anti-cancer activity
| ||
Science A subcellular map of translational machinery composition and regulation at the single-molecule level | ||
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Mol Cell An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation | ||
Nucleic Acids Res The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis |
