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验证数据展示

  • WB analysis using 30973-1-AP (same clone as 30973-1-PBS)

    WB analysis using 30973-1-AP (same clone as 30973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30973-1-AP (NLRP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

  • WB analysis using 30973-1-AP (same clone as 30973-1-PBS)

    WB analysis using 30973-1-AP (same clone as 30973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30973-1-AP (NLRP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse colon using 30973-1-AP (same clone as 30973-1-PBS)

    IP experiment of mouse colon using 30973-1-AP (same clone as 30973-1-PBS)

    IP result of anti-NLRP6 (IP:30973-1-AP, 4ug; Detection:30973-1-AP 1:1500) with mouse colon tissue lysate 1440 ug. This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 30973-1-AP (same clone as 30973-1-PBS)

    IHC staining of human colon cancer using 30973-1-AP (same clone as 30973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 30973-1-AP (NLRP6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 30973-1-AP (same clone as 30973-1-PBS)

    IF Staining of A549 using 30973-1-AP (same clone as 30973-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NLRP6 antibody (30973-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30973-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NLRP6 Polyclonal antibody, PBS Only

NLRP6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30973-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NALP6, PYPAF5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30973-1-PBS targets NLRP6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34516

Product name: Recombinant human NLRP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of NM_138329.2

Sequence: MDQPEAPCSSTGPRLAVARELLLAALEELSQEQLKRFRHKLRDVGPDGRSIPWGRLERADAVDLAEQLAQFYGPEPALEVARKTLKRADARDVAAQLQERRLQRLGLGSGTLLSVSEYKKKYREHVLQLHARVKERNARSVKITKRFTKLLIAPESAAPEEAMGPAEEPEPGRARRSDTHTFNRLFRRDEEGRRPLTVVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NLR family, pyrin domain containing 6
别名 NALP6, PYPAF5
计算分子量99 kDa
观测分子量 98-105 kDa
GenBank蛋白编号NM_138329.2
基因名称 NLRP6
Gene ID (NCBI) 171389
RRIDAB_3669800
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP59044
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOD-like receptor family pyrin domain containing 6 (NLRP6) is also named as NACHT, LRR and PYD domains-containing protein 6 and ZFML, Angiotensin II/vasopressin receptor, NALP6 and PYPAF5. NLRP6 forms a complex with ASC and caspase-1 or caspase-11 to form an inflammasome complex cleaving pro-interleukin-1β (IL-1β) and IL-18 into their biologically active forms. The function of NLRP6 has been attributed to the maintenance of epithelial integrity and host defense against microbial infections (PMID: 33910012). NLRP6 is reported to involved in inflammasome activation, regulation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, antiviral interferon (IFN) signaling, mucus secretion, and antimicrobial peptide (AMP) production. NLRP6 is a novel NLR family member, that shows high expression in the intestine and liver (in contrast to NLRP3 in myeloid cells), to regulate inflammation and host defense against microbes (PMID: 32386845).

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