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验证数据展示

  • WB analysis using 32694-1-AP

    WB analysis using 32694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32694-1-AP (NLRP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 32694-1-AP

    IHC staining of mouse lung using 32694-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32694-1-AP (NLRP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 32694-1-AP

    IHC staining of mouse lung using 32694-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32694-1-AP (NLRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NLRP4 Polyclonal antibody

NLRP4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32694-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NALP4, PAN2, PYPAF4, RNH2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32694-1-AP targets NLRP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag38545

Product name: Recombinant human NLRP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 11-200 aa of NM_134444.4

Sequence: LMWYLEELKKEEFRKFKEHLKQMTLQLELKQIPWTEVKKASREELANLLIKHYEEQQAWNITLRIFQKMDRKDLCMKVMRERTGYTKTYQAHAKQKFSRLWSSKSVTEIHLYFEEEVKQEECDHLDRLFAPKEAGKQPRTVIIQGPQGIGKTTLLMKLMMAWSDNKIFRDRFLYTFYFCCRELRELPPTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NLR family, pyrin domain containing 4
别名 NALP4, PAN2, PYPAF4, RNH2
计算分子量113kDa，994aa
观测分子量98-105 kDa
GenBank蛋白编号NM_134444.4
基因名称 NLRP4
Gene ID (NCBI) 147945
RRIDAB_3742711
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96MN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP4 is a member of the NOD-like receptor (NLR) family, a group of intracellular pattern recognition receptors (PRRs) that play crucial roles in innate immunity, inflammation, and cell death regulation.

实验方案

Product Specific Protocols
IHC protocol for NLRP4 antibody 32694-1-APDownload protocol
WB protocol for NLRP4 antibody 32694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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