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验证数据展示

  • WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

  • WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

  • IP experiment of RAW 264.7 using 85967-1-RR (same clone as 85967-1-PBS)

    IP experiment of RAW 264.7 using 85967-1-RR (same clone as 85967-1-PBS)

    IP result of anti-NLRP3 (IP:85967-1-RR, 4ug; Detection:85967-1-RR 1:1500) with RAW 264.7 cells lysate 1680 ug. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

Mouse NLRP3 Recombinant monoclonal antibody, PBS Only

NLRP3 Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 85967-1-PBS

产品说明书

CloneNo. 250083C8

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IP, Indirect ELISA

别名

AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85967-1-PBS targets NLRP3 in WB, IP, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag32515

Product name: Recombinant mouse NLRP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-153 aa of NM_145827

Sequence: MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NLR family, pyrin domain containing 3
别名 AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3
计算分子量118kd
观测分子量108-120 kDa
GenBank蛋白编号NM_145827
基因名称 Nlrp3
Gene ID (NCBI) 216799
RRIDAB_3744513
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8R4B8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.

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