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NLRP3 Monoclonal antibody
NLRP3 Monoclonal Antibody for WB,IHC,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse and More (3)
Applications
WB,IHC,ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3H1A7
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, RAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated THP-1 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68102-1-Ig targets NLRP3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications | WB,IHC,ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, Mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | NLRP3 fusion protein Ag26289 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | NLR family, pyrin domain containing 3 |
Synonyms | AGTAVPRL, AII, AII/AVP, AVP, C1orf7, Caterpiller protein 1.1, CIAS1, CLR1.1, Cryopyrin, FCAS, FCU, FLJ95925, MWS, NALP3, NLRP3, PYPAF1 |
Calculated Molecular Weight | 118 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | NM_001079821 |
Gene Symbol | NLRP3 |
Gene ID (NCBI) | 114548 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NLRP3 antibody 68102-1-Ig | Download protocol |
IHC protocol for NLRP3 antibody 68102-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Free Radic Biol Med Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis | ||
Oxid Med Cell Longev Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway | ||
Front Immunol USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis | ||
Front Pharmacol Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway | ||
Int J Mol Sci Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway | ||
J Ethnopharmacol ZeXieYin formula alleviates atherosclerosis by inhibiting the MAPK/NF-κB signaling pathway in APOE-/- mice to attenuate vascular inflammation and increase plaque stability |