验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, THP-1 cells |
| Positive IP detected in | THP-1 cells |
| Positive IF/ICC detected in | HepG2 cells |
NLRP3 expression is relatively low in normal tissues(PMID: 33538177, 35676979, 25524927, 24166187)
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27458-1-AP targets NLRP3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, Immunohistochemistry applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, ChIP, Immunohistochemistry |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, rabbit |
| 免疫原 |
CatNo: Ag26272 Product name: Recombinant human NLRP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 937-1036 aa of NM_001079821 Sequence: MLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NLR family, pyrin domain containing 3 |
| 别名 | Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein, EC:3.6.4.- |
| 计算分子量 | 118 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | NM_001079821 |
| 基因名称 | NLRP3 |
| Gene ID (NCBI) | 114548 |
| RRID | AB_2923578 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96P20 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death(PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NLRP3 antibody 27458-1-AP | Download protocol |
| IP protocol for NLRP3 antibody 27458-1-AP | Download protocol |
| WB protocol for NLRP3 antibody 27458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater 2D Indium-Vacancy-Rich ZnIn2S4 Nanocatalysts for Sonocatalytic Cancer Suppression by Boosting Cancer-Cell Pyroptosis | ||
Cell Mol Immunol The BCL-2 inhibitor APG-2575 resets tumor-associated macrophages toward the M1 phenotype, promoting a favorable response to anti-PD-1 therapy via NLRP3 activation | ||
J Adv Res Tussilagone attenuated cigarette smoke-induced chronic obstructive pulmonary disease through regulating Nrf2 and NF-κB/NLRP3 inflammasome via directly targeting cysteine 434 of KEAP1. | ||
Redox Biol Anemoside B4 alleviates ulcerative colitis by attenuating intestinal oxidative stress and NLRP3 inflammasome via activating aryl hydrocarbon receptor through remodeling the gut microbiome and metabolites. | ||
Redox Biol Betaine alleviates cognitive impairment induced by homocysteine through attenuating NLRP3-mediated microglial pyroptosis in an m6A-YTHDF2-dependent manner | ||
J Nanobiotechnology Probiotics alleviate chronic ethanol exposure-induced anxiety-like behavior and hippocampal neuroinflammation in male mice through gut microbiota-derived extracellular vesicles |
